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KMID : 0350519920450041485
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Journal of Catholic Medical College
1992 Volume.45 No. 4 p.1485 ~ p.1499
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Effects of Glycerol and Dimethylsulfoxide on the Thermotolerance of HL-60 Cells
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Abstract
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Intriguing aspects of thermal biology are the finding that both individual cells in culture and organized tissues in vivo show a marked resistance to a thermal challenge after a prior thermal exposure and that heat shock induces the synthesis of
a
set
of cellular proteins or heat shock proteins (HSPs) in mammalian systems. The phenomenon of thermotolerance is of great importance in the desing of clinical protocols using treatment with hyperthermia. But the mechanisms of the development of
thermotolerance remains undefined. To define the mechanism of the induction of thermotolernce, we reviewed the survival rate of HL-60 cells and synthesis of HSPs in HL-60 cells when added the chemicals such as glycerol or dimethylsulfoxide(DMSO)
which
changed the liquidity of cell membrane before heating.
@ES The results were as follows;
@EN 1. There were no changes in cell survival rates before and after heat treatment using varying degrees of concentration of glycerol or DMSO. Significant change of HSPs at 5% glycerol or 5% DMSO was observed.
2. Singificant changes of survival rates and synthetic appearances of HSPs in HL-60 cells were observed during the 1st hours of treatment with 5% glycerol and DMSO prior to heat at 43℃ for 30 minutes.
3. There was thermosensitivity within 2 hours but thermotolerance is increased after 2 hours, pretreatment with 5% glycerol or 5% DMSO for 1 hour before heating.
4. There were differences of synthetic appearance of HSPs after treatment with 5% glycerol and 5% DMSO. HSP110, HSP72 and HSP70 reflect the appearance of thermotolerance by heat treatment. But HSP 95 was nonspecific.
Through this study, it is shown that 5% glycerol and 5% DMSO induce thermotolerance in HL-60 cells. And thermotolerance can be evaluated by the changes of the synthetic patterns of HSPs besides the survival rate of cells and the synthesis of
HSPs.
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KEYWORD
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